FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284251
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAA150.002200486470.18095458
TTAACGG350.001331651939.5152135
ACGGGAC451.0127772E-438.24859216
GCGGTAC556.978549E-637.9403144
ACGTTGT650.002044913136.2023270
GTATCAA7850.035.1247671
GTGGTAT1900.034.466181
TTAGTGT400.002623617634.417686
GGTATCA4000.031.0195621
CGCGGTA450.00445414330.89236543
TAACGGC450.0045557230.75035336
TTTAACG450.00457941930.7177734
TATCAAC9000.030.6150342
CCTATAG450.004667150330.5988753
ATCAACG9050.030.429823
TCAACGC8950.030.379854
TGGTATC1950.030.0262832
CAACGCA9300.029.2365235
AACGCAG9800.027.7448656
CGCAGAG9850.027.6040258
Produced by FastQC (version 0.11.5)