FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530877
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19400.36543304757975953No Hit
GTATAATGACTGGCCTCTAACGTGGACGTTCGGCCAAGGGACCAAGGTGG18740.35300078926003575No Hit
GTCATTATACTGCTGACAGTAATAAAGTGCAAAATCTTCAGACTGGAGGCTGCTGATGGTGAGAGTGAACT14610.2752049909866127No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATACA14440.27200274263153235No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14400.271249272430337No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT14370.27068416977944043No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13850.2608890571639005No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11920.2245341199562234No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11830.22283881200353375No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11330.21342043448859152No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11080.2087112457311204No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGC10510.1979742953640862No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10400.1959022523107989No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10210.19232326885512085No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGTTGGCTTTTT10020.18874428539944282No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA9760.18384672909167282No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9630.18139795093778785No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9390.17687712973061556No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT9370.17650039463001788No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9220.1736748813755352No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8630.16256119590790333No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8560.1612426230558114No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGG8500.16011241775401835No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCT8480.15973568265342067No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG8480.15973568265342067No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8300.15634506674804147No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8270.15577996409714492No Hit
GTATATTACTGTGCGAAAGATATGGTAGTAAAGGTGTTTGAAGGTGTTTT8110.15276608329236338No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTCCACGTTAGAGGCCAGTCATTATA8060.15182424554086915No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7910.1489987322863865No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7830.14749179188399572No Hit
GTATGTGCCAGCACCACTCCCAGTAATAGCTGAGACCCACTCCAGCCCCTTCCCAGGAGCCTGGCGGACCCAGCT7720.14541974883070843No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTTAGCCTGGTA7660.14428954352891538No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTCCAGTCTGAAGATT7600.1431593382271223No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7580.1427826031265246No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7540.1420291329253292No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT7530.14184076537503038No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7510.1414640302744327No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7490.14108729517383498No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA7380.1390152521205477No Hit
GAGTTCACTCTCACCATCAGCAGCCTCCAGTCTGAAGATTTTGCACTTTATTACTGTCAGCAGTATAATGACTGG7280.13713157661755926No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7210.13581300376546734No Hit
CTCCAGCCCCTTCCCAGGAGCCTGGCGGACCCAGCTCATGGCGTGGCTGC7170.13505953356427194No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT7070.1331758580612835No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT6940.1307270799073985No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT6920.13035034480680083No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG6890.12978524215590428No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6840.12884340440441006No Hit
CAGTAATAAAGTGCAAAATCTTCAGACTGGAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA6830.12865503685411123No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6830.12865503685411123No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCCACGCCATGAGCTGGGTCCGCCAGGCTCCTGGGAAGGGG6780.127713199102617No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6740.1269597289014216No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6740.1269597289014216No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA6670.1256411560493297No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6650.125264420948732No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6640.12507605339843317No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6550.12338074544574357No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6440.12130870239245625No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6430.12112033484215741No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6300.11867155668827242No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6160.11603441098408859No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC6050.1139623679308013No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6040.11377400038050246No Hit
GGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACATGATGTATCTG5980.11264379507870939No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGACATAGTGATGACGCAGTC5970.11245542752841055No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA5850.11019501692482439No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA5780.10887644407273248No Hit
CCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGCTAAG5720.1077462387709394No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGTTGGC5720.1077462387709394No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT5670.10680440101944519No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTCCACGTTAGAGG5670.10680440101944519No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5630.1060509308182498No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT5560.10473235796615789No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC5540.1043556228655602No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5500.10360215266436482No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5490.10341378511406597No Hit
GCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGCTAAGCTGCTGCTA5460.10284868246316944No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG5450.1026603149128706No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT5440.10247194736257176No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5390.10153010961107753No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG5380.10134174206077869No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5360.10096500696018097No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGG5310.10002316920868674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTGT300.003049638464.63541470
CATTTAA452.1039067E-457.45369770
CGGATTT200.0073298651.7981336
AATAGTT200.007428969751.62249414
CCATTTA453.4905213E-545.8270969
GGTATCA3650.041.5884131
GTATCAA9300.041.5477641
GCTTGTA503.839261E-641.3993761
GTATTAG350.001347260339.4279751
TCTGGAG6750.036.38734470
TATCAAC10750.035.5958332
TATAAGA501.8685055E-434.4734422
CTCTGGT400.002620603734.4312119
ATCAACG11350.033.3851853
TCAACGC11500.032.9248924
CAGTTCG6100.032.84750770
CAACGCA11600.032.6349075
AACGCAG11600.032.6349076
AGAGTAC12750.029.9612911
GGGCAGT2100.029.5709841
Produced by FastQC (version 0.11.5)