FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651460
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG200.007411836651.6539735
TCGTATG404.8542977E-543.3410640
GGTATCA9200.041.5605281
GTATCAA21900.041.0527531
TATCAAC25500.035.5163272
TCAACGC25700.034.9719544
ATCAACG25850.034.769023
CAACGCA26150.034.370145
AACGCAG26450.034.11056
ACGCAGA28700.031.4339227
CGCAGAG28900.030.8589468
GTGGTAT3050.030.4936751
AGAGTAC29250.030.48969511
CAGAGTA30000.029.95700610
GCAGAGT31550.028.266999
CGTATGC658.362464E-426.69411741
GAGACCG658.723901E-426.50142324
AGTACTT14100.025.64185113
GAGTACT14300.025.52401512
TGGTATC3800.025.3738822
Produced by FastQC (version 0.11.5)