FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633158
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22480.3550456600090341No Hit
GAGTTACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCAT20070.31698249094222924No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18830.2973981217958235No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16070.25380710659898476No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15540.2454363681735049No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15330.24211966049548453No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15310.2418037835737683No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14790.23359098360914654No Hit
CTCCTGATCTGTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATT14760.23311716822657222No Hit
GTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGTAGTGGATCTGGGACAGATTTCACTCTCA14420.2277472605573964No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13570.21432249138445694No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12890.20358267604610542No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12690.20042390682894315No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAGAT12130.19157935302088894No Hit
ACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGA11790.18620944535171316No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11720.1851038761257064No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11640.1838403684388415No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC11330.17894427615224004No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11120.1756275684742197No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10900.17215292233534127No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10470.16536156851844247No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10090.15935990700583424No Hit
ATGTAACTCTGTTGACAGTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC9740.15383206087580037No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9720.15351618395408412No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9680.15288443011065167No Hit
GGGTGATGTAACTCTGTTGACAGTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT9540.15067329165863813No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9410.1486200916674827No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9260.146251014754611No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9090.1435660609200231No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT8890.1404072917028609No Hit
ATGCAGCACAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAGATAGATATTAATGCT8630.13630089172055002No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8510.13440563019025267No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8230.12998335328622557No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC8010.1265087071473471No Hit
TCTTACTACTGTCAACAGAGTTACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAA7930.12524519946048224No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCC7860.12413963023447544No Hit
GTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA7830.12366581485190109No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7800.12319199946932678No Hit
CCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCC7670.12113879947817134No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATATTGCATCTTACTACTGTCAACAGAGTTACATCACC7620.12034910717388077No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7510.11861178410444155No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7210.1138736302786982No Hit
GAGTGAAATCTGTCCCAGATCCACTACCACTGAATCTTGATGGGACCCCA7040.11118867644411032No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAATATCTATCTAAACTGGTATCAGCAGAAA6970.11008310721810355No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAATAT6900.10897753799209677No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6760.1067663995400832No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6760.1067663995400832No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6730.10629258415750886No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6690.10566083031407643No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6680.10550289185321832No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6660.10518701493150209No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6430.10155443033176552No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6430.10155443033176552No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT6370.10060679956661686No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC6370.10060679956661686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCG350.00690150652.58941770
TCGTATG352.2234935E-549.4648140
GTGGTAT1800.045.919241
GTATCAA13600.044.0622141
ATACCGT350.001240441240.0971461
TATCAAC15850.038.0246052
ATCAACG16200.036.352733
TCAACGC16200.036.352734
TGTTAGA4800.035.7900270
CAACGCA16450.035.5908975
AACGCAG16800.034.8494226
CGTATGC501.8169839E-434.6391241
CTCGTAT501.828892E-434.6006439
AGAGTAC17600.033.07229211
CGCAGAG18150.032.2573178
ACGCAGA18500.031.6470457
GCAGAGT18450.031.5461449
TGTACCG450.00466103130.6128275
GAGTACG17950.029.35737612
AGTACGG18000.029.27582613
Produced by FastQC (version 0.11.5)