FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737866
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22200.041.5348931
GGTATCA9700.037.5980531
TATCAAC25650.035.9483262
TCAACGC25600.035.884144
ATCAACG26050.035.1321873
CAACGCA26200.035.0623665
AACGCAG26600.034.276426
ACGCAGA28100.032.4445237
CGCAGAG28800.031.655948
CAGAGTA29450.030.84043310
AGAGTAC29300.030.76348311
GCAGAGT30200.030.3023669
AGTACGG25400.028.58310713
GAGTACG25250.028.47843612
TCGCTAT854.51099E-628.34184324
TACGATA500.00783705227.5246144
GTACGGG27250.026.1375314
TACGGGC5800.025.50945315
TACGGGA8900.024.35629515
GTGGTAT3850.024.1287171
Produced by FastQC (version 0.11.5)