FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737866
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8420.11411286060070529No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG208.4997606E-569.0202935
GGTATCA8100.039.9916461
GTATCAA17700.039.7176321
AATCGGG400.002416095735.00811454
CGGTAGT400.00262328434.42579329
TCGGTAG501.8874735E-434.41644728
TATCAAC20400.034.4117772
TATCGTG400.002647830934.3604745
ATCAACG20650.033.625373
CAACGCA21150.032.9795535
TCAACGC21250.032.8288084
AACGCAG21600.032.6106346
CGATTGT800.00473737330.50887570
ACGCAGA23150.030.42727
AGAGTAC23550.029.9103911
CGCAGAG23350.029.8722748
CAGAGTA24350.029.20992910
GCAGAGT25150.028.0075449
TATACGA500.007693874627.62879836
AATCGAG757.074076E-427.46939369
Produced by FastQC (version 0.11.5)