FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578876
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5900.10192165506947948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3200.042.97912
GGTATCA8900.042.5329781
GTATCAA21750.040.504551
GTCTACG350.001364079239.3291931
GTGGTAT3600.038.2367171
TATCAAC25700.034.2494962
ATCAACG27050.032.379473
CAACGCA27250.032.007485
TCAACGC27300.031.9543784
AACGCAG27300.031.9488566
ACGCAGA28700.030.3903757
CGCAGAG29000.030.075998
AGAGTAC29300.029.88524611
CAGAGTA30100.029.09095410
GCAGAGT31250.028.0204079
ATACTAA756.357977E-527.4709856
ACGGGTA2950.025.6129716
AGTACGG28150.025.2508613
GAGTACG28350.025.07272512
TACGGGA9850.023.01266915
Produced by FastQC (version 0.11.5)