FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785733
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG200.006529164553.33794458
TCGTATG404.9543494E-543.19283340
CGCTAAT405.2340845E-542.79269432
GTATCAA21900.039.5512241
ATCAACG25500.034.9036373
TCAACGC25750.034.8296284
TATCAAC25550.034.7018622
AACGCAG26050.034.559436
CAACGCA26250.034.426035
TCGCTAA501.9591348E-434.19919631
GGTATCA8900.033.3349341
ACGCAGA28450.031.8817427
TTAACGG1359.094947E-1230.54158435
AGAGTAC29600.030.41269711
CGGGTAA450.004889171630.31608617
CAGAGTA29900.030.22159610
CGCAGAG30100.030.020798
AGTACGG25650.028.8516313
GCAGAGT31400.028.7778879
CTCGTAT605.409598E-428.75998139
Produced by FastQC (version 0.11.5)