FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785733
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24450.038.4424671
GGTATCA10950.033.3985061
TCAACGC28050.032.9561234
ATCAACG28250.032.8561023
GTGGTAT4800.032.754731
AACGCAG28450.032.3687486
CAACGCA28600.032.3182375
TATCAAC29050.032.0891882
TGGTATC5100.030.798522
ACGCAGA30200.030.4930767
TTAGCGC450.004885096630.3212554
AGAGTAC30550.030.14372611
CAGAGTA31500.029.12635610
CGCAGAG31850.029.0204588
GCAGAGT33300.027.7568059
TTAACGG1750.027.47624635
AGTACGG28100.027.18855513
GTATTAG3550.026.9579641
GAGTACG29050.026.41683812
TTTAACG1850.025.96109834
Produced by FastQC (version 0.11.5)