FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449626
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9290.20661616543527286No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG7760.17258788415260684No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5930.13188739085373177No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5620.12499277177031579No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG5560.12365832936707397No Hit
GTTTTGGCCAGGGGACCAAGTTGGACATCAAACGAACTGTGGCTGCACCA5550.12343592229986701No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAAGTATTTGAATTGGTATCAGCACAAA5300.11787574561969283No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5260.11698611735086493No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5140.11431723254438132No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5020.11164834773789772No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4670.10386410038565384No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT4670.10386410038565384No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT4540.10097280851196327No Hit
GGCCAAAACTGTACGGGAGACTAGAATAATGTTGACAGTAATATGTTGCA4530.10075040144475632No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT4520.10052799437754935No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC4500.1000831802431354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT200.00732159551.81163813
GCCGTTA306.270446E-446.0547915
GTATCAA10700.041.9798161
CGCTTAA350.001320154439.59000441
CCGTTAA350.00133908539.47553316
GTGGTAT1150.039.0594861
TATCAAC12100.037.4082072
CAATCGT400.002474363434.8355255
GAGTTAG400.00257534134.552621
ATCAACG13050.034.4202353
TCAACGC13150.034.1584854
CAACGCA13200.034.0290955
AACGCAG13350.033.6467486
ACGCAGA14450.031.314077
ATCGTTT450.00437470131.0101257
AGAGTAC14650.030.88657611
ATTCGCC450.00456299430.74422331
AACTACG450.004585413730.7134382
TACCGCG450.004592906730.7031928
CGCAGAG15200.029.9962128
Produced by FastQC (version 0.11.5)