FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956826
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGG33340.3484437086784849No Hit
GTCCTGGGCCCAGTCTGGGCTGACTCAGCCACCCTCAGCGTCTGCGACCC32790.34269553711960166No Hit
GTGCAGGGTCCTGGGCCCAGTCTGGGCTGACTCAGCCACCCTCAGCGTCT28810.30109967747531946No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26610.27810699123978655No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTAAATGGTGTGGTGTTCGG26440.2763302836670408No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACCATTTAGGCTGTCATCCCATG24860.25981735446152177No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACCATTTAGG23880.24957515786569345No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20630.21560868956320167No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGGGCTGACTCAGCCAC20200.2111146645262566No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19610.20494844412672736No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19310.2018130778218819No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18750.19596039405283722No Hit
TTCCTGGGAGGTGCTGATACCAAGTAACAGTTTTACTTCCGATGTTGAAGCTGCCTCCAGAACAAGAGATGATGA18120.18937612481266186No Hit
TCCCAGGAATGGCCCCCAAACTCCTCATGTATAAAAATTTTCAGCGGCCC17740.18540466082652435No Hit
GTATCAGCACCTCCCAGGAATGGCCCCCAAACTCCTCATGTATAAAAATTTTCAGCGGCCCTCAGGGGTCCCTGA17280.18059709915909478No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17000.17767075727457238No Hit
CCCTCAGCGTCTGCGACCCCCGGGCAGAGCGTCATCATCTCTTGTTCTGG16610.17359478107827336No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16070.16795112172955168No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15990.16711502404825956No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15660.16366612111292964No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14870.15540965651017008No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14540.15196075357484015No Hit
CCCCTGAGGGCCGCTGAAAATTTTTATACATGAGGAGTTTGGGGGCCATTCCTGGGAGGTGCTGATACCAAGTAA14340.1498705093716099No Hit
GTCTGGGCTGACTCAGCCACCCTCAGCGTCTGCGACCCCCGGGCAGAGCGTCATCATCTCTTGTTCTGGAGGCAG14280.1492434361106408No Hit
CCATTTAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTC13980.1461080698057954No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13240.13837416625384344No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12720.13293953132544475No Hit
AGTCTGGGCTGACTCAGCCACCCTCAGCGTCTGCGACCCCCGGGCAGAGC12600.1316853848035066No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12590.13158087259334508No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12540.13105831154253753No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGT12490.13053575049172994No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12220.1277139208173691No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12090.12635526208526943No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11830.12363794462107008No Hit
GCATGGGATGACAGCCTAAATGGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG11800.12332440799058554No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG11760.12290635914993948No Hit
GCCTAAATGGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA11730.12259282251945494No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11690.12217477367880888No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGTCCAC11560.12081611494670923No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11430.11945745621460957No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT11190.11694916317073324No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11160.1166356265402487No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACCATTTAGGC11040.11538148001831053No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10670.11151452824233454No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10390.10858818635781219No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10210.10670696657490496No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10180.10639342994442041No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA10150.10607989331393587No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10060.10513928342248224No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10050.10503477121232073No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10010.10461672237167469No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9610.10043623396521417No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGTCCACTGATG9570.10001818512456812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2800.046.8335571
GTATCAA19250.040.1558531
ACGCGTC400.002594817734.5035312
TATCAAC22650.034.1244772
TCAACGC23450.032.6660274
ATCAACG23700.032.321453
CAACGCA23650.032.097985
AACGCAG24300.031.0973956
ACGCAGA25550.029.5744517
ATCGCGG850.00605772629.01394570
CGCAGAG26350.028.6765568
AGAGTAC26250.028.65435811
CAGAGTA27050.027.93446510
GCAGAGT29050.026.2488129
AGTACGG22250.024.81152513
TACGGGG11500.024.60251415
GAGTACG22600.024.42727712
TACGTGT851.4420525E-424.355437
GAGTACT8750.023.65956112
AGTACTT9200.022.87733713
Produced by FastQC (version 0.11.5)