FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872858
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4250.048.503212
GGTATCA14250.044.605651
GTATCAA38650.043.025791
GTGGTAT5300.042.137671
TATCGGA600.001411455939.05610370
TATCAAC45200.036.864762
ATCAACG48300.034.6409653
AACGCAG48500.034.1439256
TCAACGC49000.034.0759774
CAACGCA49550.033.697745
ACGCAGA51450.032.1843647
CGCAGAG52300.031.6612918
AGAGTAC52250.031.6258411
CAGAGTA53850.030.68616710
GCAGAGT55600.029.7821129
GTTATAC1103.81242E-828.1098143
AGTACGG40000.027.4851513
GAGTACG40050.027.36505312
TACGGGC4750.026.76339115
GAGTACT22700.024.67004412
Produced by FastQC (version 0.11.5)