FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975188
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28550.043.4530681
GGTATCA10800.039.8850631
TATCAAC32000.038.4452132
GTGGTAT3200.037.6913831
ATCAACG32850.037.4504363
CAACGCA33200.037.1594285
TCAACGC33500.036.8266564
AACGCAG35600.034.5574956
ACGCAGA36900.033.3383147
CAGAGTA37450.032.94071210
CGCAGAG37850.032.5925948
AGAGTAC38500.031.95282711
TGGTATC3600.031.588972
AACGCGT450.004133654831.37565
GCAGAGT40600.030.4698439
ACGGGTA2200.029.76303916
CTCGCTA1202.7175702E-928.71577523
CGCGTAG500.006513064328.58885667
TCGTATG500.00757334827.71922140
GTCTATA756.210596E-527.5685561
Produced by FastQC (version 0.11.5)