FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232337
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGATTTTCGG54020.4383541190437356No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG52680.4274804700337651No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCATCTGGGACCC48840.3963201624231034No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAATCACACCATTCAGGCTGTCATCCCATG46610.37822446295128687No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43350.3517706601359855No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAATCACACCATTCAGG40570.32921189577201687No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCATCT40230.3264529102023229No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36160.2934262300003976No Hit
GTGCTGACTCAGCCACCCTCAGCATCTGGGACCCCCGGGCAGAGGGTCAC35630.2891254583770511No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC35240.28596073963534324No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34920.28336404733445475No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG33090.26851421323874886No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32920.2671347204539018No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC32270.2618601892177221No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30570.24806526136925208No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29840.24214155705785026No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG29640.24051862436979496No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG28710.23297198737033784No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA26160.2122795955976328No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25800.20935831675913327No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25520.20708621099585584No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25490.20684277109264756No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24540.1991338408243849No Hit
GCATGGGATGACAGCCTGAATGGTGTGATTTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG24240.19669944179230192No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGATTTTCGGCGGAGGGAC24060.19523880237305216No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22890.18574464614792868No Hit
GTTATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG22860.18550120624472038No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22840.18533891297591487No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22520.1827422206750264No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22430.1820119009654015No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22290.1808758480837628No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22100.17933406203011026No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21780.1767373697292218No Hit
CTCTTGTTCTGGAGGCGACTCCAACGTCGGCAGAAATGCTGTCAACTGGTACCAGCAGCTCCCAGGAACGGCCCC21250.17243659810587525No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT21150.1716251317618476No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21030.17065137214901444No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT20800.16878499955775084No Hit
GTCTGACACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG20330.16497110774082088No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACC20280.16456537456880707No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20150.16351046832157112No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC19710.15994001640784947No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA19660.15953428323583566No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18520.15028356691392047No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18360.14898522076347623No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18280.1483360476882541No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18150.14728114144101817No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17910.1453336222153518No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17780.14427871596811587No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC17500.14200661020483846No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT17110.1388418914631306No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC17060.13843615829111677No Hit
GTTTGGTCCCTCCGCCGAAAATCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCT16890.1370566655062698No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAATCACACCATTCAGGC16810.13640749243104766No Hit
GTGCAGCATGGGATGACAGCCTGAATGGTGTGATTTTCGGCGGAGGGACC16700.13551487945261725No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG16590.13462226647418685No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAATCACAC16400.13308048042053433No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16350.1326747472485205No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16290.1321878674421039No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15890.1289420020659933No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC15850.12861741552838224No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15850.12861741552838224No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15800.12821168235636843No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15720.1275625092811463No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTC15670.12715677610913248No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG15560.12626416313070207No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15550.12618301649629932No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15240.1236674708298136No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14880.12074619199131406No Hit
GGGTCACCATCTCTTGTTCTGGAGGCGACTCCAACGTCGGCAGAAATGCT14720.11944784584086983No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG14530.1179060597872173No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCATCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAGGCGA14180.11506592758312051No Hit
GTAGTAGACGGCCATGTCGTCAGACTTCAACCTGCTCAACTCCATGTAGGCTGTGCTGATGGACGTGTCCGTGGT14130.1146601944111067No Hit
CCCTCAGCATCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG13670.11092744922857951No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC13600.11035942278776016No Hit
CTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGAGAATCGGTC13360.10841190356209382No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13240.10743814394926063No Hit
GGATGACAGCCTGAATGGTGTGATTTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC13140.10662667760523298No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCATCTGGGACCCCCGGGCAGAGGGTCAC13100.10630209106762194No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGTTATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG13040.10581521126120533No Hit
ATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAG12980.10532833145478875No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12870.10443571847635834No Hit
CCCCTGAGGGCCGCTGATTATAACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTGA12760.10354310549792792No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAG12730.10329966559471963No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAAAAT12720.10321851896031686No Hit
GGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG12610.10232590598188644No Hit
CCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGAT12500.10143329300345603No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG12490.10135214636905326No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12380.10045953339062287No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTA200.005467826555.78600368
TACGACT200.0069907852.42645358
GTATCAA27000.044.05431
TATCAAC30150.039.1069682
GTGGTAT3900.038.013411
GGTATCA11450.037.3378371
ATCAACG32550.036.2235033
TCAACGC32550.036.2235034
CAACGCA33000.035.729545
TAACGAG400.002451459234.90645656
AACGCAG33950.034.62826
AGAGTAC36800.032.50718311
CGCAGAG36650.032.45218
CAGAGTA37000.032.23829710
ACGCGTA751.6753165E-632.1853227
ACGCAGA37300.031.6093067
GCAGAGT38150.031.3568659
TGGTATC5000.028.9597382
CGTATGC854.305759E-628.51454241
TCGTATG854.3166965E-628.50524940
Produced by FastQC (version 0.11.5)