FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727659
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22600.041.4338341
ATCCGGT350.001360745939.35014323
ATCAACG25700.036.0316353
TCAACGC25800.035.891984
CAACGCA26150.035.5432325
GGTATCA9400.035.1591261
AACGCAG26500.035.0737886
CGATTAA851.6922777E-435.05734670
TATCAAC26550.034.8780822
ACGCAGA27050.034.2333837
AGAGTAC27950.033.2542211
CGCAGAG27850.033.2500238
CAGAGTA28000.033.1948410
TACGCTG1154.7293724E-1132.9592829
GCAGAGT30000.031.0965989
GTGGTAT3700.030.7048441
TGGTATC3850.029.5065232
AGTACGG26100.028.48905613
GAGTACG26300.028.40330112
ATTACGT500.00772772727.60391233
Produced by FastQC (version 0.11.5)