FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727659
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8270.11365213650899667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22600.040.8718571
GGTATCA9650.038.573981
CAACGCA26600.034.482035
ATCAACG26600.034.378873
TTATATC400.002647999234.3599664
TCAACGC26700.034.2312774
TATCAAC27000.033.906862
AACGCAG27600.033.232686
ACGCAGA28450.032.1190387
CGCAGAG29150.031.3477448
CAGAGTA29450.030.91176410
AGAGTAC29350.030.90003611
GCAGAGT30800.029.5568649
GTGGTAT3300.029.2443561
AGTACGG25850.028.70492213
GAGTACG26450.028.1836512
TGGTATC3600.026.7685722
TAGTCGG1057.968738E-726.1736265
GTACGGG30000.024.50505814
TACGGGT6000.024.05032315
Produced by FastQC (version 0.11.5)