FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756061
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16460.21770730139499325No Hit
CTCCAGACCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCAAAACTAC15930.21069728500742665No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG15800.20897784702557068No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15220.20130650833729025No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14760.19522234317072298No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12960.17141474034502507No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12550.16599189747917165No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11700.15474941836703654No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11680.15448488944675098No Hit
GTTTTGGCCAGGGGACCAAGTTGGACATCAAACGAACTGTGGCTGCACCA11110.1469458152186133No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10990.14535864169690013No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10620.1404648566716178No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10420.13781956746876245No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10360.13702598070790584No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9950.13160313784205244No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9850.13028049324062477No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9740.12882558417905435No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9690.1281642618783405No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9680.12803199741819773No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9660.1277674684979122No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9510.1257835015957707No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT9080.12009612980963176No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAAGTATTTGAATTGGTATCAGCACAAA8890.1175831050669192No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8730.11546687370463495No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT8660.11454102248363558No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8660.11454102248363558No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGCTGAAAGTAA8550.11308611342206515No Hit
GCACAAACCAGGGCAAGCCCCTAAGCTCCTGATCTACGATGTATCCAATT8540.11295384896192238No Hit
GGCCAAAACTGTACGGGAGACTAGAATAATGTTGACAGTAATATGTTGCA8400.11110214651992366No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC8390.1109698820597809No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8360.11057308867935259No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8340.11030855975906707No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT8260.10925044407792492No Hit
GTGTATTATTGTGCAAGACCCCGGCAGGATTATTATTCTTACTTCGGTAT8120.10739874163592619No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG8040.10634062595478407No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8020.10607609703449854No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8010.10594383257435577No Hit
AACCAGGGCAAGCCCCTAAGCTCCTGATCTACGATGTATCCAATTTGGAA7800.10316627891135768No Hit
GGTTTGTGCTGATACCAATTCAAATACTTGCTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACT7780.10290174999107214No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT7730.10224042769035832No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAACTTGGTCCCCTGGCCAAAACTG7670.10144684092950174No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7600.10052098970850236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGC200.00735114951.76230624
GTATCAA12850.041.6136671
GTGGTAT2350.036.7011451
TATCAAC15350.034.609162
GTGTAAG807.090421E-834.4990731
ATCAACG15650.033.945733
TCAACGC15950.033.3072474
CAACGCA16300.032.3804245
AACGCAG16500.032.1970066
GTCTAGC553.191356E-431.47102738
AGAGTAC17400.030.33338511
CGCAGAG18100.029.350868
CAGAGTA18400.029.059810
CGAAATT605.178326E-428.97535748
ACGCAGA18900.028.1084987
CGTAGGG500.0075782727.71473739
ATGCGTA500.00763493727.67246636
GTATTAG1005.015063E-727.5992571
TATTAGG500.00773678227.5974332
TGCTACG500.00773678227.5974332
Produced by FastQC (version 0.11.5)