FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756061
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17270.22842072266655733No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14740.19495781425043746No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG14720.19469328533015193No Hit
CTCCAGACCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCAAAACTAC14390.19032855814544064No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14360.18993176476501233No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13600.17987966579416212No Hit
GTTTTGGCCAGGGGACCAAGTTGGACATCAAACGAACTGTGGCTGCACCA11430.15117827794318184No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11340.14998789780189695No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10660.14099391451218882No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10510.13900994761004734No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10350.13689371624776306No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10080.13332257582390838No Hit
GGCCAAAACTGTACGGGAGACTAGAATAATGTTGACAGTAATATGTTGCA9870.1305450221609103No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9770.12922237755948263No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9690.1281642618783405No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9300.12300594793277263No Hit
GTGTATTATTGTGCAAGACCCCGGCAGGATTATTATTCTTACTTCGGTAT9230.12208009671177326No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9180.12141877441105943No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9150.12102198103063112No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9090.12022839426977454No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT8860.11718631168649091No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8720.11533460924449217No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8620.11401196464306451No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGCTGAAAGTAA8500.11242479112135131No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8450.11176346882063748No Hit
AACCAGGGCAAGCCCCTAAGCTCCTGATCTACGATGTATCCAATTTGGAA8390.1109698820597809No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8380.11083761759963812No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8370.11070535313949537No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAAGTATTTGAATTGGTATCAGCACAAA8300.109779501918496No Hit
GCACAAACCAGGGCAAGCCCCTAAGCTCCTGATCTACGATGTATCCAATT8220.10872138623735388No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8210.1085891217772111No Hit
GGTTTGTGCTGATACCAATTCAAATACTTGCTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACT8190.10832459285692556No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAACTTGGTCCCCTGGCCAAAACTG8160.10792779947649726No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7920.10475345243307088No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT7840.10369533675192875No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7750.10250495661064385No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT7680.10157910538964449No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7670.10144684092950174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13950.045.790671
TATCAAC16050.039.767732
ATCAACG16650.038.0845533
TCAACGC16750.037.6290254
CAACGCA17150.036.751385
AACGCAG17300.036.4327286
AGAGTAC18450.034.3485311
CGCAGAG18900.033.530718
CAGAGTA19150.032.9131210
ACGACTC552.8982802E-431.99205864
ACGCAGA20050.031.60757
ATATATC450.00464643130.6332243
CGATAAA450.004649440330.62917324
GCCGTTA605.4721E-428.70345715
TAACGGC854.349582E-628.47499736
TTAACGG854.3675973E-628.45987935
CCTATAC756.2067076E-527.5699043
GAGTACG19000.027.37222312
AGTACGG18950.027.26269313
TACACGA658.652584E-426.539382
Produced by FastQC (version 0.11.5)