FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583969
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10250.037.667361
GTATCAA23900.036.803961
TATCAAC27300.032.958192
ATCAACG27350.032.6524283
TCAACGC27350.032.5296754
CAACGCA27600.032.2350235
AACGCAG28000.031.774526
GTGGTAT4250.031.598191
TGGTATC4150.031.5506042
CGCAGAG28750.030.945628
ACGCAGA28850.030.8383587
AGAGTAC29400.030.37564311
CGTAGAC450.005279134529.8427373
TCGTATG703.975983E-529.42957340
CAGAGTA30350.029.3142210
CGCTAAT704.410678E-528.98540132
GCAGAGT31100.028.6072869
GAGTACG25350.026.62007712
AGTACGG25550.026.41169713
CGTATGC809.6960364E-525.818741
Produced by FastQC (version 0.11.5)