FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583969
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9780.16747464334579404No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA250.001470119577.6923370
GGTATCA9650.039.1215551
GTATCAA24000.038.4826281
TATCAAC26900.033.783352
ATCAACG27550.032.932583
TCAACGC27800.032.861284
CAACGCA27650.032.788195
AACGCAG27750.032.6700336
TGGTATC3700.031.8388632
ACGCAGA29050.031.323627
AGAGTAC29200.030.93272811
CGCAGAG29350.030.8890428
GTGGTAT4150.030.8646131
CAGAGTA30350.030.0925510
GCAGAGT31000.029.3532649
AGTACGG26200.028.06671513
GAGTACG26750.027.48964112
TTATACC500.00881364926.8689294
TACGGGG9400.026.0761715
GTACGGG28800.025.29973814
Produced by FastQC (version 0.11.5)