FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738604
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21350.040.7879451
CAACGCA22900.038.1749085
TCAACGC23500.037.200234
ATCAACG23700.037.031533
AACGCAG24250.036.475496
TATCAAC24650.036.023222
AGAGTAC25600.034.54730211
GCTTACG400.002626376534.4175342
ACGCAGA26150.033.691377
CAGAGTA27050.032.6954110
CGCAGAG26950.032.5613488
CCTATAC751.6996255E-632.123033
GGTATCA8850.031.8918511
GCAGAGT28950.030.4307299
GTGGTAT3900.029.1245041
AGTACGG24900.028.746513
GAGTACG25150.028.46074912
TGGTATC4200.027.8618142
ACGGGTA1254.256435E-927.53216616
CGATTGT900.009611580526.39392570
Produced by FastQC (version 0.11.5)