FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860367
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35550.043.8131521
GGTATCA13650.040.8063961
TGGTATC4950.040.2404752
TATCAAC40350.038.4711532
ATCAACG42450.036.465873
TCAACGC42750.036.117084
CAACGCA43000.035.905015
AACGCAG44050.035.0491646
GTGGTAT6050.033.5306931
AGAGTAC47950.032.77086311
CGCAGAG47900.032.375318
ACGCAGA47750.032.333317
CAGAGTA49100.031.72381410
GCAGAGT50350.030.868099
CGAATTA1052.4572728E-829.40962615
GAGTACT17150.028.80775612
AGTACGG38650.027.51840413
GAGTACG39300.027.23786512
AGTACTT18100.027.10619213
CCGAATT1155.947004E-826.85070614
Produced by FastQC (version 0.11.5)