FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093284
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20080.1836668239908386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15050.040.6370661
GTATCAA37250.040.2665181
CAACGCA45200.033.571635
TCAACGC45050.033.5335584
ATCAACG45050.033.468583
AACGCAG45900.032.9853556
TATCAAC46500.032.370832
TGGTATC5450.031.9405122
ACGCAGA48200.031.4113667
CGCAGAG50150.030.1899858
AGAGTAC50450.029.94287111
CAGAGTA50750.029.90025110
CTCGCTA1600.029.86594623
GTGGTAT5900.029.5341171
GCAGAGT53250.028.560529
AGTACGG42050.027.6528113
GAGTACG42500.027.36001412
GTACGGG46150.024.97444714
TACGGGG18600.024.93313415
TACGGGT8050.022.8743515
Produced by FastQC (version 0.11.5)