FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287087
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC200.00739129351.682841
GGTATCA3250.041.346271
GTATCAA8200.041.1781961
ATCAACG9250.036.8764043
CAACGCA9300.036.6781435
TCAACGC9500.035.905974
TATCAAC9600.035.531952
AACGCAG9650.035.347856
ACGCAGA10250.033.272917
CAGAGTA10500.032.48069410
AGTACGG8750.031.89013913
AGAGTAC10700.031.8735811
ACTGTCG553.2791888E-431.3174748
GAGTACG8950.031.17751112
CGCAGAG11200.030.4506538
GCAGAGT11250.030.3153179
TACGGGT1800.028.70768715
GTACGGG9850.028.32880214
TAGACCT500.007774521627.564185
GTATAAG658.706982E-426.504021
Produced by FastQC (version 0.11.5)