FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223312
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT78210.6393299501680683No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56330.46047124527512195No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48130.39344010358763754No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46800.38256797938710646No Hit
GATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGCATCTGGGACAGATTTCA46510.38019736583962227No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG46310.3785624599448056No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46170.37741802581843387No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45150.3690800057548687No Hit
GAGTTACACTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT43710.3573086833121885No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41690.34079613377453993No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40150.32820735838445136No Hit
ACACAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT38790.31708999829969786No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38620.31570032828910366No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTC37960.3103051388362086No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG37600.30736230822553856No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAACTATGTAAATTGGTATCAACACAAA35610.2910949945721124No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC35250.2881521639614424No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAA34950.28569980511921733No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG34740.2839831539296598No Hit
CCATAGATCAGGAGCTTAGGGGCTTGCCCTGGTTTGTGTTGATACCAATT33600.27466419032920464No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32760.26779758557097455No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31550.2579064049073336No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT31240.25537230077036766No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31230.2552905554756268No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC31220.255208810180886No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30690.25087630955962176No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30120.24621682775939416No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28860.235916920622049No Hit
AACCAGGGCAAGCCCCTAAGCTCCTGATCTATGGTGCATCCAATTTGCAA28360.23182965588500726No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27500.2247995605372955No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26820.2192408804949187No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26410.21588932341054448No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26310.21507187046313614No Hit
GGTTTGTGTTGATACCAATTTACATAGTTGTTAATGTTCTGACTTGCCCGGCAAGTGATGGTGACT26230.21441790810520947No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25870.21147507749453942No Hit
ACTTACTACTGTCAACAGAGTTACACTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG25460.2081235204101652No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCCCAGATGCACTGCCA25160.20567116156794016No Hit
CCCCTAAGCTCCTGATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCA25120.2053441803889768No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC24380.19929502857815506No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23900.195371254430595No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23750.19414507500948244No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGTGTAACT23450.19169271616725742No Hit
GTTCAGTGGCAGTGCATCTGGGACAGATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTA23280.1903030461566632No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22720.18572530965117648No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA22670.1853165831774723No Hit
GATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTA22490.18384516787213728No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22450.18351818669317393No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22190.18139280902991223No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT21530.17599761957701715No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21250.17370875132427377No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC21250.17370875132427377No Hit
GGTGATGGTGAGAGTGAAATCTGTCCCAGATGCACTGCCACTGAACCTTG21100.17248257190316127No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGCATCTGGGACAGATTTCACT20940.1711746471873079No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20850.17043893953464037No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20050.1638993159553736No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19060.155806531776031No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18970.15507082412336345No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18870.1542533711759551No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA18600.15204624821795257No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACT18370.15016610643891337No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT18270.14934865349150503No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17990.14705978523876165No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17810.14558836993342664No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC17610.14395346403860992No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17350.14182808637534824No Hit
ATCTGGGACAGATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATT17310.1415011051963849No Hit
CATCAAGGTTCAGTGGCAGTGCATCTGGGACAGATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTG17020.1391304916489007No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16980.13880351046993733No Hit
GCTTAGGGGCTTGCCCTGGTTTGTGTTGATACCAATTTACATAGTTGTTA16980.13880351046993733No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16700.13651464221719398No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16670.13626940633297147No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16650.1361059157434898No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16620.1358606798592673No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16520.13504322691185897No Hit
GAGTGAAATCTGTCCCAGATGCACTGCCACTGAACCTTGATGGGACCCCA16300.1332448304275606No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16180.1322638868906706No Hit
AGTCAGAACATTAACAACTATGTAAATTGGTATCAACACAAACCAGGGCA16080.13144643394326222No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15800.12915756569051887No Hit
ACCTTGATGGGACCCCACTTTGCAAATTGGATGCACCATAGATCAGGAGC15490.12662346155355297No Hit
GTCCCAGATGCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATT15430.12613298978510798No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15420.12605124449036714No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15410.1259694991956263No Hit
GCCCTGGTTTGTGTTGATACCAATTTACATAGTTGTTAATGTTCTGACTTGCCCGGCAAGTGATGGTGACT15390.12580600860614463No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGA14980.12245445152177041No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14760.12065605503747204No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14740.12049256444799038No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGATGCACTGCCACTGAACCTTGA14710.12024732856376788No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14560.11902114914265535No Hit
GGGCAAGCCCCTAAGCTCCTGATCTATGGTGCATCCAATTTGCAAAGTGG14200.1160783185319853No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14180.11591482794250363No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14140.1155878467635403No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT14110.1153426108793178No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14060.11493388440561361No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13960.11411643145820527No Hit
CTCCTGATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT13930.11387119557398277No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13880.11346246910027859No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGA13870.11338072380553776No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13850.11321723321605609No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13610.11125534614227606No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAAC13450.1099474214264227No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG13360.10921171377375519No Hit
ATGTAAATTGGTATCAACACAAACCAGGGCAAGCCCCTAAGCTCCTGATC13320.10888473259479184No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13250.10831251553160601No Hit
GCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGT13230.10814902494212433No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13170.10765855317367931No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG13060.10675935493153015No Hit
GTATCAACACAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATGGTGCAT12910.10553317551041763No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12760.1043069960893051No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12590.10291732607871092No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAACTATGTAAATTGGTATCAACACAAACCAGGGCAAG12590.10291732607871092No Hit
GATCAGGAGCTTAGGGGCTTGCCCTGGTTTGTGTTGATACCAATTTACAT12480.10201812783656172No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12330.1007919484154492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)