FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223312
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT74900.6122722576088521No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47130.385265574113554No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46480.3799521299553998No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG46320.3786442052395464No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45910.37529264815517216No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44930.3672816092705704No Hit
GATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGCATCTGGGACAGATTTCA42850.35027858796447675No Hit
GAGTTACACTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT42590.3481532103012151No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41910.3425945302588383No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40110.327880377205488No Hit
ACACAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT39180.3202780647945904No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36390.29747112756189753No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTC36100.29510051401441334No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAACTATGTAAATTGGTATCAACACAAA34670.283410936866474No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG34170.2793236721294322No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAA32740.2676340949814929No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG32680.26714362321304785No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32320.26420079260237783No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC32290.2639555567181553No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC31950.2611762166969669No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31730.2593778202126686No Hit
CCATAGATCAGGAGCTTAGGGGCTTGCCCTGGTTTGTGTTGATACCAATT31090.2541461213492551No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30430.24875093189636005No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30270.24744300718050669No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT29860.24409145009613248No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29640.2422930536118341No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT29020.23722484533790236No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27500.2247995605372955No Hit
AACCAGGGCAAGCCCCTAAGCTCCTGATCTATGGTGCATCCAATTTGCAA26740.21858691813699205No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26120.2135187098630603No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25810.2109846057260944No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25710.21016715277868606No Hit
GGTTTGTGTTGATACCAATTTACATAGTTGTTAATGTTCTGACTTGCCCGGCAAGTGATGGTGACT25680.20992191689446354No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25500.20845050158912853No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25320.2069790862837935No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24960.20403625567312345No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCCCAGATGCACTGCCA24590.2010116797677126No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24370.1992132832834142No Hit
CCCCTAAGCTCCTGATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCA24340.19896804739919172No Hit
ACTTACTACTGTCAACAGAGTTACACTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG24180.19766012268333832No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC23570.19267365970414743No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGCATCTGGGACAGATTTCACT23460.19177446146199822No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA23140.18915861203029155No Hit
GTTCAGTGGCAGTGCATCTGGGACAGATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTA21740.17771427076657467No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21550.17616111016649882No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21390.17485318545064546No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21300.17411747779797793No Hit
GGTGATGGTGAGAGTGAAATCTGTCCCAGATGCACTGCCACTGAACCTTG21230.1735452607347921No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC21190.17321827955582877No Hit
GATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTA21130.17272780778738375No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGTGTAACT20800.17003021306093621No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT20750.16962148658723203No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20670.16896752422930536No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20310.1660246936186353No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20140.16463502360804114No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18820.15384464470225095No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18570.15180101233373006No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA18310.14967563467046838No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACT18110.14804072877565166No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17640.14419869992283244No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT17220.14076539754371736No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17190.14052016165949488No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17180.14043841636475404No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC16990.1388852557646782No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16570.13545195338556312No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16320.13340832101704225No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16210.13250912277489307No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16170.13218214159592973No Hit
GAGTGAAATCTGTCCCAGATGCACTGCCACTGAACCTTGATGGGACCCCA16130.1318551604169664No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16100.1316099245327439No Hit
GCTTAGGGGCTTGCCCTGGTTTGTGTTGATACCAATTTACATAGTTGTTA16070.13136468864852138No Hit
GCCCTGGTTTGTGTTGATACCAATTTACATAGTTGTTAATGTTCTGACTTGCCCGGCAAGTGATGGTGACT15940.13030199981689056No Hit
ATCTGGGACAGATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATT15550.127113933321998No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15480.12654171625881214No Hit
AGTCAGAACATTAACAACTATGTAAATTGGTATCAACACAAACCAGGGCA15350.12547902742718128No Hit
CATCAAGGTTCAGTGGCAGTGCATCTGGGACAGATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTG15270.12482506506925461No Hit
GTCCCAGATGCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATT15260.12474331977451378No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15040.12294492329021542No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14870.12155525327962123No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14870.12155525327962123No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14790.12090129092169455No Hit
GTATCAACACAAACCAGGGCAAGCCCCTAAGCTCCTGATCTATGGTGCAT14740.12049256444799038No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGATGCACTGCCACTGAACCTTGA14700.12016558326902703No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGA14600.11934813032161869No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14510.11861242266895118No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14490.1184489320794695No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14450.11812195090050617No Hit
ACCTTGATGGGACCCCACTTTGCAAATTGGATGCACCATAGATCAGGAGC14400.11771322442680199No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14310.11697751677413448No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14240.11640529971094864No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13990.11436166734242778No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13840.11313548792131525No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGA13730.11223628967916607No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13510.11043789319486771No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13480.1101926573106452No Hit
CTCCTGATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT13410.10962044024745934No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAACTATGTAAATTGGTATCAACACAAACCAGGGCAAG13250.10831251553160601No Hit
GGGCAAGCCCCTAAGCTCCTGATCTATGGTGCATCCAATTTGCAAAGTGG13190.107822043763161No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13170.10765855317367931No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13100.10708633611049348No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13010.10635062845782597No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13000.10626888316308514No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12990.1061871378683443No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12910.10553317551041763No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG12890.10536968492093596No Hit
ATGTAAATTGGTATCAACACAAACCAGGGCAAGCCCCTAAGCTCCTGATC12700.1038165243208601No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT12500.1021816184260434No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG12410.10144591077337589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)