FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584792
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA19070.3260988522414807No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA18860.3225078318444849No Hit
GGGTACGACGGGCCCCTGGACAAGGGCTTGAGTGGATGGGTTGGATCAACCCTAACAGTGGTGCCACAAACTATG18740.32045582018905866No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCTGCTGTCCCATGCCTGACAGTAATAGTCAG18250.31207677259606836No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18070.30899875511292907No Hit
CCCCTGTGCTGGTCATTGATCAAGATAACAAGCGGCCCTCAGGGATCCCT17930.30660474151493183No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14510.24812240933528504No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14380.2458993967085733No Hit
CCCAAAGGCTGCTGTCCCATGCCTGACAGTAATAGTCAGCCTCATCCATA14190.24265037825414848No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14110.24128237048386433No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC13720.2346133326037292No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13600.23256132094830298No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13370.22862829860873607No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC12560.21477721993460921No Hit
GTATACATGGAGCTGAGCAGGCTGAGATCTGACGACACGGCCGTGTATTA12490.21358021313561062No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12490.21358021313561062No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12270.20981819176732924No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12230.20913418788218716No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12020.2055431674851913No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG11740.20075514028919683No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11720.2004131383466258No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG11450.19579611212191686No Hit
CCATGTATACTGTGCTGATGGACGTGTCCCTGGTCATGAGGACCCAGCCCTGAAACTTCTGTGCATAGTTTGTGG11390.19477010629420374No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCATGGGACAGCAGCCTTTGGGTGTTCGGCGGAGGGACCAAG11320.19357309949520513No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11230.19203409075363548No Hit
GCACAGGGGACTGGCCTGGCTTCTGTTGATACCAGCAAGCATATTTATCC11190.19135008686849342No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT11070.1892980752130672No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG11060.18912707424178168No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10930.18690406161506998No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10860.18570705481607136No Hit
GACTATTACTGTCAGGCATGGGACAGCAGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG10850.18553605384478586No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10830.1851940519022148No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10800.18468104898835827No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG10720.18331304121807412No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAGCTGACT10540.18023502373493483No Hit
GTGCTGGTCATTGATCAAGATAACAAGCGGCCCTCAGGGATCCCTGAGCG10330.17664400333793895No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10190.1742499897399417No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10170.17390798779737068No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT9950.1701459664290893No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9760.1668969479746645No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9700.1658709421469514No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9480.16210892077867No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9390.16056991203710036No Hit
GTCCCATGCCTGACAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGC9310.1592019042668162No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9240.15800489746781762No Hit
CTATTACTGTCAGGCATGGGACAGCAGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC9120.15595288581239142No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9080.15526888192724933No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8990.1537298731856797No Hit
GCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC8970.15338787124310865No Hit
GGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAC8940.1528748683292521No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8830.15099385764511142No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8790.15030985375996936No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8750.14962584987482727No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT8620.1474028372481156No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8610.14723183627683004No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8540.14603482947783145No Hit
GGCCAGTCCCCTGTGCTGGTCATTGATCAAGATAACAAGCGGCCCTCAGG8430.14415381879369074No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCATGGGACAGCAGC8160.1395367925689818No Hit
CTGTTAGGGTTGATCCAACCCATCCACTCAAGCCCTTGTCCAGGGGCCCG8160.1395367925689818No Hit
GTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAG8100.13851078674126868No Hit
GCACAGGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCC8090.13833978576998318No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8040.1374847809135556No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA7900.13509076731555836No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7880.13474876537298733No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7790.13320975663141768No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7660.13098674400470595No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7520.1285927304067087No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT7480.12790872652156665No Hit
ATAATACACGGCCGTGTCGTCAGATCTCAGCCTGCTCAGCTCCATGTATACTGTGCTGATGGACGTGTCCCTGGT7480.12790872652156665No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG7420.12688272069385353No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7380.12619871680871148No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA7360.12585671486614042No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT7320.1251727109809984No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA7250.12397570418199975No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7220.1234627012681432No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7080.12106868767014596No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC7010.11987168087114736No Hit
GTCGTACCCAGTGCAGATAGTAGCCGGTGAAGGTGTATCCAGAAGCCTTG6990.1195296789285763No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6900.11799067018700667No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6880.11764866824443562No Hit
CCCTAACAGTGGTGCCACAAACTATGCACAGAAGTTTCAGGGCTGGGTCCTCATGACCAGGGACACGTCCATCAG6870.11747766727315011No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT6840.11696466435929355No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA6830.11679366338800805No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCTGCTGTCCCATGCCTGACAGTAATAGTCAGC6670.11405764784743978No Hit
AGCCAGGCCAGTCCCCTGTGCTGGTCATTGATCAAGATAACAAGCGGCCC6630.11337364396229771No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAG6630.11337364396229771No Hit
GTATCAACGCAGAGTACGGGGAGCATCACCCAGCAACCACATCTGTCCTCTAGAGAATCCCCTGAGAGCTCCGTT6570.1123476381345846No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA6550.11200563619201356No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6510.1113216323068715No Hit
GTATCAACGCAGAGTACGGGGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTT6400.10944062162273083No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT6390.10926962065144531No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA6380.10909861968015977No Hit
GTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAA6320.10807261385244668No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG6320.10807261385244668No Hit
CATCTGTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACT6300.10773061190987565No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC6240.10670460608216253No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCTG6210.106191603168306No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG6200.10602060219702047No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC6140.10499459636930737No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6130.10482359539802186No Hit
GGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGATAAATA6060.10362658859902324No Hit
CTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGA6040.10328458665645221No Hit
CAATAATACACGGCCGTGTCGTCAGATCTCAGCCTGCTCAGCTCCATGTA6010.10277158374259565No Hit
GCTTGGTCCCTCCGCCGAACACCCAAAGGCTGCTGTCCCATGCCTGACAGTAATAGTCAGCCTCATCCATAGC6010.10277158374259565No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG5970.1020875798574536No Hit
GGACAGCAGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT5900.10089057305845497No Hit
GGATGAGGCTGACTATTACTGTCAGGCATGGGACAGCAGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGT5870.10037757014459842No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA5870.10037757014459842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)