FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584792
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTACGACGGGCCCCTGGACAAGGGCTTGAGTGGATGGGTTGGATCAACCCTAACAGTGGTGCCACAAACTATG19160.32763786098305037No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18070.30899875511292907No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA17710.3028427201466504No Hit
CCCCTGTGCTGGTCATTGATCAAGATAACAAGCGGCCCTCAGGGATCCCT17400.2975416900367994No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA17280.2954896783813732No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCTGCTGTCCCATGCCTGACAGTAATAGTCAG16590.28369061136267254No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15390.2631704948084105No Hit
GTATACATGGAGCTGAGCAGGCTGAGATCTGACGACACGGCCGTGTATTA14790.25291043653127954No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13200.2257212820968823No Hit
CCCAAAGGCTGCTGTCCCATGCCTGACAGTAATAGTCAGCCTCATCCATA13170.22520827918302574No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12730.21768423644646304No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12660.2164872296474644No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12450.21289620925046857No Hit
CCATGTATACTGTGCTGATGGACGTGTCCCTGGTCATGAGGACCCAGCCCTGAAACTTCTGTGCATAGTTTGTGG12440.21272520827918304No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCATGGGACAGCAGCCTTTGGGTGTTCGGCGGAGGGACCAAG12110.20708217622676098No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12080.20656917331290442No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12040.20588516942776236No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12010.2053721665139058No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11920.20383315777233613No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC11870.20297815291590854No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11660.19938713251891274No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11620.19870312863377063No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG11540.1973351208634865No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG11390.19477010629420374No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG10850.18553605384478586No Hit
GACTATTACTGTCAGGCATGGGACAGCAGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG10800.18468104898835827No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG10700.18297103927550307No Hit
GTGCTGGTCATTGATCAAGATAACAAGCGGCCCTCAGGGATCCCTGAGCG10640.18194503344778998No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT10580.1809190276200769No Hit
GCACAGGGGACTGGCCTGGCTTCTGTTGATACCAGCAAGCATATTTATCC10510.17972202082107827No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10430.17835401305079412No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10280.17578899848151136No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10170.17390798779737068No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10100.17271098099837207No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAGCTGACT9770.16706794894595No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9630.16467393534795277No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9590.1639899314628107No Hit
CTGTTAGGGTTGATCCAACCCATCCACTCAAGCCCTTGTCCAGGGGCCCG9560.16347692854895415No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9550.16330592757766865No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9500.16245092272124106No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT9450.16159591786481348No Hit
GGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAC9390.16056991203710036No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9310.1592019042668162No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9140.15629488775496245No Hit
GCACAGGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCC9060.1549268799846783No Hit
GCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC9040.1545848780421073No Hit
CTATTACTGTCAGGCATGGGACAGCAGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC8980.15355887221439418No Hit
GTCCCATGCCTGACAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGC8950.15304586930053762No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8860.15150686055896798No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8520.1456928275352604No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8430.14415381879369074No Hit
ATAATACACGGCCGTGTCGTCAGATCTCAGCCTGCTCAGCTCCATGTATACTGTGCTGATGGACGTGTCCCTGGT8390.14346981490854868No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8340.1426148100521211No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8320.14227280810955006No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8300.14193080616697903No Hit
GGCCAGTCCCCTGTGCTGGTCATTGATCAAGATAACAAGCGGCCCTCAGG8270.14141780325312248No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8260.14124680228183698No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT8240.14090480033926592No Hit
GTCGTACCCAGTGCAGATAGTAGCCGGTGAAGGTGTATCCAGAAGCCTTG8230.14073379936798042No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8210.1403917974254094No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8100.13851078674126868No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8060.13782678285612662No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8060.13782678285612662No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA7820.1337227595452742No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7720.13201274983241904No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7710.13184174886113353No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7620.1303027401195639No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7560.12927673429185077No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCATGGGACAGCAGC7500.12825072846413765No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAG7330.1253437119522839No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA7330.1253437119522839No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7150.12226569446914459No Hit
CCCTAACAGTGGTGCCACAAACTATGCACAGAAGTTTCAGGGCTGGGTCCTCATGACCAGGGACACGTCCATCAG7100.121410689612717No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7020.12004268184243286No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT6920.1183326721295777No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG6910.11816167115829218No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6770.11576765756029495No Hit
CAATAATACACGGCCGTGTCGTCAGATCTCAGCCTGCTCAGCTCCATGTA6730.11508365367515287No Hit
GTATCAACGCAGAGTACGGGGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTT6700.11457065076129631No Hit
GGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGATAAATA6640.11354464493358322No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA6600.11286064104844115No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA6590.11268964007715564No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT6580.11251863910587012No Hit
GGACAGCAGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT6560.11217663716329908No Hit
GTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAG6550.11200563619201356No Hit
AGCCAGGCCAGTCCCCTGTGCTGGTCATTGATCAAGATAACAAGCGGCCC6510.1113216323068715No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT6490.11097963036430047No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6470.11063762842172943No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT6450.1102956264791584No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC6420.10978262356530184No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA6380.10909861968015977No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCTGCTGTCCCATGCCTGACAGTAATAGTCAGC6370.10892761870887427No Hit
GTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAA6270.10721760899601909No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT6260.10704660802473359No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT6250.10687560705344808No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6110.10448159345545084No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCTG5980.1022585808287391No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5920.10123257500102599No Hit
AGATAACAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCAT5890.10071957208716946No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5880.10054857111588394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT100.009732796130.4086570
GTGGTAT1150.063.049611
TACTATG5400.039.84708870
TATTACC350.001347734639.425832
GTATTAG459.9633115E-538.3635181
TAAGGCT451.0089691E-438.28154
ATCAACG12600.038.037343
CAACGCA12600.037.73145
TCAACGC12650.037.5854764
TAAATGA1403.5897756E-837.25961370
AACGCAG12900.037.120986
CGCAGAG13350.035.8697138
ATAATCC501.8666868E-434.479893
AGAGTAC14050.034.0826111
GCAGAGT14150.033.841749
CAGAGTA14300.033.48675510
ACGCAGA14550.032.9113857
TACATGC652.3206821E-531.843942
CAACGGA802.408955E-630.71667964
AACGGCC450.00458959430.70923637
Produced by FastQC (version 0.11.5)