FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294155
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48800.041.8401261
GGTATCA19700.039.4092451
TATCAAC58150.035.287692
TCAACGC58200.035.2573784
ATCAACG58550.035.046613
CAACGCA59650.034.4576535
AACGCAG61350.033.614336
ACGCAGA64100.032.1684887
CGCAGAG64800.031.926538
AGAGTAC66950.030.90125811
CAGAGTA68050.030.4017510
GCAGAGT69850.029.6183139
GTGGTAT6450.029.163621
CGCATAC605.5176084E-428.66474335
CTCGTAT1004.9764094E-727.62659539
AGTACGG51850.026.71022813
GAGTACG52550.026.35443112
TCGTATG1208.288771E-825.94852840
TACGGGT13150.025.48522615
TGGTATC7250.025.4728432
Produced by FastQC (version 0.11.5)