FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294155
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20530.15863633026955812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48900.040.7241671
GGTATCA21050.037.223861
TATCAAC58000.034.3524252
ATCAACG59400.033.6854023
TCAACGC59200.033.668234
AACGCAG60150.033.133926
CAACGCA60400.032.9402735
ACGCAGA63000.031.5808337
CGCAGAG63450.031.2492878
AGAGTAC64850.030.6272911
CAGAGTA66500.029.81604810
GCAGAGT67500.029.323779
ACGGGTA3900.027.1337716
AGTACGG53400.027.09693513
GAGTACG54000.026.79585812
GTGGTAT6800.026.6688941
TAACGGC2350.026.37095636
TTAACGG2400.025.79943835
TACGGGT14000.025.59806815
ACGGGCT5300.024.47489216
Produced by FastQC (version 0.11.5)