FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586876
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC200.007237171351.9657940
CGATTGT505.341057E-447.57505870
TATACGA350.001331152739.52525336
TTATACG350.001335637339.49822235
GTATCAA14400.038.994411
GTGGTAT2350.038.1138421
GCCTACG557.4635154E-637.580781
GGTATCA7050.034.693371
CCGTTAA501.8765371E-434.4490516
TATCAAC17450.031.9813542
CAACGCA17600.031.9045165
ATCAACG18300.030.495883
TCAACGC18350.030.4127854
TGGTATC3000.029.8558442
AACGCAG19000.029.553666
TACTCGA500.006758248428.3714466
ACGCAGA20150.027.8669747
AGAGTAC20200.027.62745511
CGCAGAG20200.027.6274558
CAGAGTA20350.027.42381510
Produced by FastQC (version 0.11.5)