FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856044
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGAGTCCTTCAGTGACTACTACTGGAACTGGCTCCGCCAGCC30510.35640691366331634No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25980.30348907299157524No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20080.23456738205045535No Hit
CATCTATAGTGCATCCACCAGGGTCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA18330.21412450761876725No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17200.20092425155716293No Hit
GTCTGGGACAGAGTTCACTCTCTCCATCAGCAGCCTGCAGTCTGAAGATT15850.18515403413843215No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15690.18328497133324922No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14750.17230422735279963No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13940.16284209690156112No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13690.15992168626846281No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13420.1567676427847167No Hit
GAGTTCACTCTCTCCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATGATAATTGG13350.15594992780744915No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12980.15162772007046366No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12700.14835686016139357No Hit
GTATGATAATTGGCCTAAGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT12440.14531963310297133No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTTAGCAG12170.14216558961922518No Hit
CTCCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATGATAATTGGCCTAAGACGTT12160.14204877319390125No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGACCCTGGT11890.13889472971015507No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11690.13655840120367643No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11620.13574068622640892No Hit
CTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGC11330.1323530098920149No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTTGGCCGAACGTC11250.13141847848942345No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT11100.12966623210956446No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10730.12534402437257897No Hit
GTAATATAAAGCCGTGTCCGCGGCGGTCACAGAGTTCAGTTTCAGGGAGA10660.12452630939531145No Hit
CTCTTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT9990.11669960889860802No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9920.1158818939213405No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTA9850.11506417894407296No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC9840.11494736251874904No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9730.11366238184018577No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9540.11144286975903109No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9070.10595249776880628No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8740.10209755573311653No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8580.10022849292793362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGATTG9750.044.5574470
GGTATCA7500.040.5022961
GTATCAA14750.036.9762881
TAGTACC652.3503284E-531.7873744
ATCAACG17500.031.101983
TCAACGC17650.031.0219544
CAACGCA17700.030.92895
GTGGATT9450.030.67709269
TATCAAC17650.030.6639862
AACGCAG18250.030.3741176
TACGGGC3150.029.51512115
AGAGTAC19950.028.1310111
CGCAGAG20300.027.81568
TAACGTT500.007797196527.55388523
ATAGCGG1154.676258E-827.52422163
AATAGCG1154.7417416E-827.48486362
ACGCAGA20500.027.0403697
GCAGAGT21800.025.9016829
AGTGGAT10350.025.64725368
CAGAGTA21800.025.58580810
Produced by FastQC (version 0.11.5)