FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006239
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT10170.10106942783970807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24250.040.6253551
GGTATCA9950.040.5047231
TCGTATG1950.039.10350440
TCAACGC27500.035.8188364
CAACGCA27850.035.3686945
CGCTAAT2150.035.31585732
TCGCTAA2150.035.3105831
ATCAACG28100.034.808893
AACGCAG28300.034.806296
TATCAAC28400.034.8050082
CTCGTAT2400.031.74619739
GCTAATC2400.031.65603633
CGCAGAG31700.031.181778
ACGCAGA32050.030.7337917
AGAGTAC33150.030.02565211
CGTATGC2700.029.5413741
CAGAGTA34200.029.30522210
GCAGAGT34200.029.0031079
CTTATAC3550.028.1392521
AGTACGG27200.026.71724713
Produced by FastQC (version 0.11.5)