FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006239
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21820.21684709099925567No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA350.001278525639.85362251
GTATCAA25900.038.4944731
GGTATCA10950.035.6011961
TCAACGC29350.034.0142634
CAACGCA29650.033.5495
TATCAAC29850.033.1331142
ATCAACG30100.032.949463
AACGCAG31000.032.0879946
ACGCAGA32850.030.2809097
AGAGTAC33500.029.48787911
CGCAGAG34050.029.3148278
CAGAGTA34650.028.70787210
GCAGAGT35850.027.9389619
TATAACG658.1667653E-426.80431649
GTATACG658.6585194E-426.5373121
AGTACGG30100.026.07202513
AATAGCG2350.025.46624862
GAGTACG31050.025.38518312
TTAACGG2050.025.28184535
TAACGGC2100.024.69097936
Produced by FastQC (version 0.11.5)