FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016289
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54160.5329192778825708No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37190.3659392161088037No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36630.36042897246747724No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33270.32736751061951863No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32850.3232348278885238No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA32830.32303803347276216No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31640.3113287657349435No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30800.30306340027295386No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27080.2664596389412854No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26890.26459009199154965No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25690.2527824270458502No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25390.24983051080942528No Hit
GCCTAGAACCTGAAGATTTTGCAGTTTATTACTGTCAGCACCGTAGCGAC24870.24471385599962214No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24710.24313950067352888No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGGG24250.23861322911101074No Hit
GTTTATTACTGTCAGCACCGTAGCGACTGGTCCGCGCTCACTTTCGGCGG23980.23595650449822836No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23790.2340869575484926No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23560.23182382176723354No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCCCTCCGCCGAAAGTGAGCGCGGACCAGTCGCTACGGTG23490.23113504131206772No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22670.22306647026583976No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21920.21568667967477753No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21460.2111604081122594No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGGGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT21400.21057002486497442No Hit
GTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGGGCTACTTAGCCTGGTA20620.20289504265026975No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19920.1960072380986117No Hit
GTGTTAGGGGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC19390.19079218608092777No Hit
GTCTGGGACGGACTTCAGTCTCACCATCAGCAGCCTAGAACCTGAAGATT19180.18872584471543036No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCC19160.1885290502996687No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19110.18803706426026454No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18810.18508514802383969No Hit
GTAATAAACTGCAAAATCTTCAGGTTCTAGGCTGCTGATGGTGAGACTGAAGTCCGTCCCAGACCCACTGCCA17120.16845601989197956No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16940.16668487015012462No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16900.1662912813186013No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16630.16363455670581892No Hit
GACTTCAGTCTCACCATCAGCAGCCTAGAACCTGAAGATTTTGCAGTTTA16260.15999386001422825No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15990.15733713540144584No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15820.15566438286747175No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15360.1511381113049536No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15230.14985894760250282No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15000.14759581182124376No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14870.146316648118793No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14650.14415190954541474No Hit
ACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC14460.14228236259567897No Hit
CCGTAGCGACTGGTCCGCGCTCACTTTCGGCGGAGGGACCAAGGTGCAGATCAAACGAACTGTGGCTGCACCAT14340.14110159610110903No Hit
GTTCTAGGCTGCTGATGGTGAGACTGAAGTCCGTCCCAGACCCACTGCCACTGAACCTGGGTGGGATGCCAGTGG13990.13765769382528No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT13620.13401699713368934No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13620.13401699713368934No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13340.13126187531302613No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13300.1308682864815028No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13300.1308682864815028No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13160.1294907255711712No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC13120.12909713673964787No Hit
TTATTACTGTCAGCACCGTAGCGACTGGTCCGCGCTCACTTTCGGCGGAGGGACCAAGGTGCAGATCAAACGAA13100.1289003423238862No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13060.1285067534923629No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGCGGACCAGTCGCTACGGTGCTGA13020.12811316466083958No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12920.1271291925820313No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACATCCGAGAGGGCCACTGGCATCCCACCCAGGTT12850.1264404121268655No Hit
GTGCAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC12620.12417727634560641No Hit
GAGTGTTAGGGGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACATC12510.1230949070589173No Hit
CTCCCAGGCTCCTCATCTATGATACATCCGAGAGGGCCACTGGCATCCCA12460.12260292101951314No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12450.12250452381163232No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCCGGGGAAAGAGC12330.12132375731706237No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12260.12063497686189656No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12210.12014299082249243No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12190.11994619640673078No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11730.11541992484421264No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11490.11305839185507272No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11460.11276320023143023No Hit
CAGTAATAAACTGCAAAATCTTCAGGTTCTAGGCTGCTGATGGTGAGACTGAAGTCCGTCCCAGACCCACTGCCA11450.1126648030235494No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11350.11168083094474113No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11350.11168083094474113No Hit
GTCAGAGTGTTAGGGGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATA11260.11079525607381364No Hit
GTCCCAGACCCACTGCCACTGAACCTGGGTGGGATGCCAGTGGCCCTCTC11170.10990968120288619No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11060.10882731191619707No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10960.10784333983738877No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10890.10715455938222299No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10730.1055802040561297No Hit
GCACCGTAGCGACTGGTCCGCGCTCACTTTCGGCGGAGGGACCAAGGTGC10670.10498982080884474No Hit
GGACCAAGGTGCAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10580.10410424593791726No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10560.10390745152215561No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10510.10341546548275148No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10500.10331706827487062No Hit
GGCCACTGGCATCCCACCCAGGTTCAGTGGCAGTGGGTCTGGGACGGACT10440.10272668502758567No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10330.10164431574089654No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10240.10075874086996907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)