FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270627
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3690.1363500315933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAC200.007203389452.0196141
ACGTGAT350.00753489351.42069670
GGTATCA3300.043.884331
GTATCAA8250.042.630491
AGTCACG350.001368272239.2971158
TATCAAC9700.036.2176252
ATCAACG9850.035.6133583
TCAACGC9900.035.4269454
GGACTAA400.002571776534.5573838
CGAAAGG400.00261659734.4358831
CAACGCA10300.034.0511445
AACGCAG10500.033.7300266
ACGGGGC1253.6379788E-1233.0095816
GGCTAAT652.0740681E-532.36186655
ACGCAGA11100.031.9067827
TGGTATC1305.456968E-1231.792852
AGAGTAC11250.031.48135811
GTGGTAT1550.031.1437171
GCCTTAC450.004629245530.6493741
CGCAGAG11600.030.5314888
Produced by FastQC (version 0.11.5)