FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302810
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTATGGTGTCCACTGGTACCAGCAGTTTCCAGGAACCGTCCCCAAACTCCTCATCTATGGTAACAACAATCGG9460.3124071199762227No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAACCACTCAGGCTGCTGTCATAGGA8720.28796935372015453No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAACCACTCAGG8430.27839239126845217No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGGTGTCCACTGGTACCAGCAGTTTCCAGGAACCGT8350.2757504705921205No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7670.2532941448433011No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAG7310.2414055017998085No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6560.21663749545919883No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6430.21234437436015982No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC6190.20441861233116476No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC5560.18361348700505267No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG5510.18196228658234537No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5380.17766916548330636No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT5220.17238532413064298No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCCGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG5190.1713946038770186No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG4990.16478980218618935No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC4780.15785476041081867No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC4620.15257091905815529No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG4580.15124995871998942No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC4500.14860803804365774No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC4480.1479475578745748No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG4410.14563587728278457No Hit
GGCCATCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTC4400.14530563719824313No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCCGTGTTCGG4310.14233347643736996No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT4300.1420032363528285No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA4120.13605891483108218No Hit
GTGTCTGGGGCCCCAGGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAG4040.1334169941547505No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA4010.1324262739011261No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG3900.12879363297117002No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA3690.12185859119579934No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAACCACTCAGGC3670.12119811102671643No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG3660.12086787094217495No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA3610.11921667051946765No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG3590.11855619035038474No Hit
CCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGACGGTGACCCACTGCCCTGGGGCCCCAGACA3530.11657474984313596No Hit
TCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCCAGTCCTAT3450.11393282916680426No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG3430.11327234899772134No Hit
GGGCAGTGGGTCACCGTCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGGTGTCCACTGGTACCAG3430.11327234899772134No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT3370.11129090849047256No Hit
AGGTTATGGTGTCCACTGGTACCAGCAGTTTCCAGGAACCGTCCCCAAAC3210.10600706713780918No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT3200.10567682705326772No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT3190.10534658696872627No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA3170.10468610679964334No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT3160.10435586671510189No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGGAAC3150.10402562663056042No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA3070.10138370595422871No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCAC150.002343440769.0772912
GATCAGT350.007299358551.83793370
TACTAGA200.007326129451.7994162
CTTCGGT306.2744773E-446.04392221
GTATCAA7500.041.4463731
GGTATCA3050.038.5020981
GTGGTAT901.200533E-1038.3762741
ATCAACG8300.037.445363
TATCAAC8350.037.2211342
TCAACGC8600.036.1391264
CAACGCA8650.035.5310065
AACGCAG8900.034.5329446
CGCAGAG9500.032.3519138
AGAGTAC9550.032.18253311
CAGAGTA9600.032.01491510
ACGCAGA9650.031.8490357
TGGCTTA450.00434877231.04453357
GCAGAGT10550.029.1320559
GTCCTAA854.375719E-628.443591
CTGCTAG500.007677783727.6354829
Produced by FastQC (version 0.11.5)