FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810727
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1700.042.80994840
GTATCAA23400.041.3340381
CGTATGC1850.041.2428941
GGTATCA9350.040.862861
GACCGAT2200.040.69636526
CTCGTAT1800.040.39649239
ATCTCGT1800.040.32892637
ACCGATC2350.038.1057827
AGGGGGG3500.038.01366470
TCTCGTA1950.035.47817638
CAACGCA28600.034.4162675
TCAACGC28750.034.3564154
ATCAACG29100.034.0614623
CCGATCA2800.033.2178528
TATCAAC29600.033.1372872
AACGCAG30000.033.039626
TATCTCG2200.032.9718836
CGATCAG2750.032.57719829
AGACCGA2750.032.55106425
ACGCAGA30750.032.34377
Produced by FastQC (version 0.11.5)