FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670912
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGACTGAAATCTGTC34160.5091576838691214No Hit
ATATTATACTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT33260.4957431078889631No Hit
CTCCTAAACTGCTCATTTCCTGGGCATCTACCCGTGAATCCGGGGTCCCT33210.49499785366784316No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAACTTGGTACCAGCAGAA33100.49335829438137935No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29720.44297910903367355No Hit
ATACTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA27630.4118274825908614No Hit
GTTTAGGAGGCTGTCCTAGTTTCTGCTGGTACCAAGTTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT25710.3832097204998569No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGACTGAAAT22310.33253243346370315No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18970.28274945149289327No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18920.28200419727177334No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18740.2793212820757417No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17640.2629256892111037No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17370.2589013164170562No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16050.2392266049794906No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTGGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA13900.20718067347133456No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG13840.20628636840599066No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGACTGAAATCTGTCCCAGACCCGCTGCCA13670.20375250405418296No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13400.19972813126013544No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCAGTCTCAC13010.19391514833540016No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13000.19376609749117618No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12820.19108318229514454No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12750.19003982638557665No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12530.18676070781264906No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12510.1864626061242011No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12470.18586640274730515No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC12410.18497209768196127No Hit
AACTAGGACAGCCTCCTAAACTGCTCATTTCCTGGGCATCTACCCGTGAA12130.1807986740436898No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11790.1757309453400744No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCACGGGT11740.1749856911189545No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11050.16470118286749977No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA10890.16231636935991606No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10740.16008060669655633No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCCACTGCAAGTCCAGCCAGAGTGTTTTATA10720.15978250500810837No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10510.15665243727940475No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG9950.14830559000286175No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGACTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC9910.14770938662596586No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9540.1421945053896785No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9500.14159830201278262No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9360.13951159019364687No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9090.13548721739959935No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCAGTCTCACCATCAGCAGCC9080.13533816655537537No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9050.13489101402270343No Hit
GTCCTAGTTTCTGCTGGTACCAAGTTAAGTAGTTCTTATTGTTGGAGCTG8990.13399670895735954No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8940.13325145473623962No Hit
GTCAGGGACCCCGGATTCACGGGTAGATGCCCAGGAAATGAGCAGTTTAGGAGGCTGTCCTAGTTTCTGCTGGTA8860.13205904798244777No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCCACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA8640.12877992940952018No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8630.12863087856529618No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8040.11983687875608127No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8030.11968782791185728No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT8030.11968782791185728No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7870.11730301440427358No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7850.11700491271582562No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGACTGAAATCTGTCCCAGACCCGCTGCCA7740.11536535342936181No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7730.11521630258513785No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7650.11402389583134598No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCGC7600.11327864161022608No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT7430.11074477725841839No Hit
ACTTAACTTGGTACCAGCAGAAACTAGGACAGCCTCCTAAACTGCTCATT7370.10985047219307449No Hit
GATTTCAGTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACT7330.10925426881617857No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA7310.10895616712773061No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7260.10821091290661071No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT7250.10806186206238672No Hit
GTCTCTGGGCGAGAGGGCCACCATCCACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTA7040.1049317943336831No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCCACTGCAAGTCCAGC7010.10448464180101116No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7010.10448464180101116No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6980.10403748926833922No Hit
GTCTGGGACAGATTTCAGTCTCACCATCAGCAGCCTGCAGGCTGAAGATG6770.1009074215396356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)