FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957840
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31930.3333542136473733No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23330.24356886327570365No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21810.2276998246053621No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21370.22310615551657895No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG20710.2162156518834043No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20570.2147540299006097No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19580.20441827445084773No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19500.20358306188925082No Hit
GTAATATGTTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCG18530.19345610957988807No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18220.1902196609037No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18050.18844483421030653No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16900.1764386536373507No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16640.17372421281216072No Hit
ACATATTACTGTCAACAGTTTCATAATTTACCGATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAA15860.16558089033659065No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15790.16485007934519336No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15640.16328405579219912No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15560.16244884323060219No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15470.16150922909880563No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG14660.15305270191263676No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14350.14981625323644868No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14330.14960745009604945No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGAAATCATCAACTATTTAAATTGGTATCAGCAGCAA14000.1461621982794621No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13720.14323895431387287No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13630.14229934018207632No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13530.14125532448008019No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13350.13937609621648708No Hit
ATATTACTGTCAACAGTTTCATAATTTACCGATCACTTTCGGCGGAGGGA13340.1392716946462875No Hit
CACCATCAGCAGCCTGCAACCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTACCGATCACTTT13170.137496867952894No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12950.13520003340850248No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12920.13488682869790364No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC12790.1335296082853086No Hit
GGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTGACGGGACCCCT12760.13321640357470976No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12640.13196358473231437No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12550.13102397060051782No Hit
CAGTAATATGTTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCG12100.12632589994153512No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCAT11970.12496867952894013No Hit
GCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTG11880.12402906539714359No Hit
GTAATACACGCCCGTGTCCTCGACTCTCAGGCTGCTCATTTGGAGATACA11570.12079261672095548No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11500.12006180572955817No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11470.11974860101895933No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11450.11953979787856009No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAATTTAAAT11230.11724296333416856No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT11220.11713856176396892No Hit
CTCCTGATCTGCGATGCATCGAAATTGGAACCAGGGGTCCCGTCAAGGTT11140.11630334920237201No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11080.11567693978117431No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT10970.11452852250897853No Hit
GTCTCCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGAAATCATCAA10940.11421531779837969No Hit
CCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCA10760.1123360895347866No Hit
ATCTTCAGGTTGCAGGCTGCTGATGGTGAGGGTAAAATTTGCCCCAGATCCACTTCCGCTGAACCTTGACGGGAC10700.11170968011358891No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10610.11077006598179237No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10600.11066566441159276No Hit
ATTTTACCCTCACCATCAGCAGCCTGCAACCTGAAGATCTTGCAACATATTACTGTCAACAGTTTCATAATTTAC10560.11024805813079429No Hit
CTCCTGTGTAGTGTCTGGCTTCACCTTTAGTGACTATTGGATGAGCTGGGTCCGCCAGGTTCCAGGGAAAGGG10410.10868203457780004No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10140.10586319218241043No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10030.10471477491021464No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9980.10419276705921658No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9840.10273114507642195No Hit
CAGTAATACACGCCCGTGTCCTCGACTCTCAGGCTGCTCATTTGGAGATA9800.1023135387956235No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9720.10147832623402656No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9640.10064311367242963No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGGTGTGCCATCCAGATGACCCAGTCTCCTTC9640.10064311367242963No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9610.10032990896183078No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9590.10012110582143156No Hit
CCCTTAGACTCTCCTGTGTAGTGTCTGGCTTCACCTTTAGTGACTATTGG9580.10001670425123194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)