FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836608
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53380.6380527080783354No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43340.5180442931456548No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37370.4466847077723378No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36980.44202302631578944No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35080.419312270501836No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31830.38046492503059975No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31260.3736516982864137No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28600.34185664014687883No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28010.33480435281517745No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27560.32942548959608325No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27250.3257200504895961No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26970.3223732022643819No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG26510.31687480875153No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26090.3118545364137087No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26000.31077876376988983No Hit
GTAGGAGACAGAGTCACCATCAGTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAAAGA25630.30635614290085683No Hit
GACCAGGGAAAGCCCCTGAGCTCCTGATCTATGAAGCATACAGTTTGCAA25510.304921779375765No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24940.298108552631579No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAGGTG24870.2972718405752754No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24100.28806800795593634No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC23240.2777884026927785No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21890.26165181303549573No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTCACGGCATC21490.2568706012851897No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21330.2549581165850673No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAGTTGCCGGGCAAGTCAGAGCATTAGCAG21120.2524479804161567No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20780.24838395042839656No Hit
ACTTACTACTGTCAACAGACTCACGGCATCCCGCCCACCTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAA20510.24515663249694003No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19890.23774575428396572No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA18520.22137010403916768No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18030.21551311964504283No Hit
AGCATACAGTTTGCAAAGCGGGGTCTCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCAT17760.2122858017135863No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17720.2118076805385557No Hit
CTGTATGCTTCATAGATCAGGAGCTCAGGGGCTTTCCCTGGTCTTTGCTGATACCAATTTAAATAGCTGCTAATG17660.2110904987760098No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17440.2084608323133415No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17290.20666787790697677No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17280.20654834761321908No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTCACGGCATCCCGCCCACCTT16580.19818122705018362No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16500.1972249847001224No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16310.19495390911872706No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15970.19088987913096694No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15830.18921645501835985No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15770.18849927325581398No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA15610.18658678855569155No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGAGACCCCG15420.1843157129742962No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTCACGGCATCCCGCCCACCTTCGGCGGAGGGA15360.18359853121175032No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCAGTTGCCGGGCAAGTCAGA15350.18347900091799266No Hit
GGGAAAGCCCCTGAGCTCCTGATCTATGAAGCATACAGTTTGCAAAGCGG15260.1824032282741738No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15230.18204463739290086No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15130.18084933445532436No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15050.17989309210526316No Hit
ACCTTGATGAGACCCCGCTTTGCAAACTGTATGCTTCATAGATCAGGAGCTCAGGGGCTTTCCCTGGTCTTTG14990.17917591034271727No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14500.1733189259485924No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14470.17296033506731945No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14270.17056972919216648No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14230.17009160801713588No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14190.16961348684210528No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14100.16853771419828642No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13800.1649518053855569No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13600.16256119951040393No Hit
GCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAGGTGGGCGG13550.16196354804161567No Hit
CCTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCA13540.161844017747858No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13460.16088777539779683No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13400.1601705936352509No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13370.15981200275397797No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG13270.15861669981640147No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13160.1573018665850673No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13140.157062805997552No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13040.15586750305997552No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12870.15383548806609548No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12830.15335736689106488No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12600.15060817013463892No Hit
GACTCACGGCATCCCGCCCACCTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT12570.15024957925336596No Hit
GCATACAGTTTGCAAAGCGGGGTCTCATCAAGGTTCAGTGGCAGTGGATC12460.14893474602203183No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG12430.1485761551407589No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12220.1460660189718482No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12150.14522930691554467No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGAGACCCCGCTTTGCAAA12120.14487071603427173No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12100.14463165544675644No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12080.1443925948592411No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12050.14403400397796817No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11990.14331682221542227No Hit
CCTTGGTCCCTCCGCCGAAGGTGGGCGGGATGCCGTGAGTCTGTTGACAG11910.14236057986536108No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11880.14200198898408814No Hit
ACTGTCAACAGACTCACGGCATCCCGCCCACCTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTG11780.14080668604651161No Hit
GCAAAGACCAGGGAAAGCCCCTGAGCTCCTGATCTATGAAGCATACAGTTTGCAAAGCGGGGTCTCATCAAGGTT11750.14044809516523868No Hit
GATCTATGAAGCATACAGTTTGCAAAGCGGGGTCTCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA11650.13925279222766218No Hit
CTCCTGATCTATGAAGCATACAGTTTGCAAAGCGGGGTCTCATCAAGGTT11590.13853561046511628No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11350.1356668834149327No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAGTTGCCG11330.1354278228274174No Hit
GACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCT11290.1349497016523868No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11250.1344715804773562No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGAGACCCCGCTTTGCAAACT11100.13267862607099143No Hit
GATCTCCACCTTGGTCCCTCCGCCGAAGGTGGGCGGGATGCCGTGAGTCTGTTGACAGTAGTAAGTTGCAAAAT11080.13243956548347613No Hit
TCCCTGGTCTTTGCTGATACCAATTTAAATAGCTGCTAATGCTCTGACTTGCCCGGCAACTGATGGTGACT11070.1323200351897185No Hit
GTCAACAGACTCACGGCATCCCGCCCACCTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCAC11030.1318419140146879No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11010.13160285342717257No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10660.12741929314565484No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCA10630.12706070226438188No Hit
AGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAAAGACCAGGGAA10610.12682164167686658No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGA10590.12658258108935128No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10350.12371385403916768No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT10330.12347479345165238No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10320.12335526315789473No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA10300.12311620257037945No Hit
GATCAGGAGCTCAGGGGCTTTCCCTGGTCTTTGCTGATACCAATTTAAAT10250.12251855110159118No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10170.12156230875152998No Hit
GGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC10000.11953029375764994No Hit
ACCTTGGTCCCTCCGCCGAAGGTGGGCGGGATGCCGTGAGTCTGTTGACA9920.11857405140758874No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9910.1184545211138311No Hit
CAGCAAAGACCAGGGAAAGCCCCTGAGCTCCTGATCTATGAAGCATACAG9830.11749827876376989No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9740.11642250611995103No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9520.11379283965728273No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9460.11307565789473685No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGC9360.11188035495716035No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT9340.11164129436964504No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9300.11116317319461444No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC9260.11068505201958384No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9100.10877256731946146No Hit
GTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCA9090.1086530370257038No Hit
GGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATC9070.10841397643818848No Hit
GTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC9060.10829444614443084No Hit
GTTTGCAAAGCGGGGTCTCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGT8880.10614290085679315No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGC8800.10518665850673195No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG8780.10494759791921664No Hit
GAGCATTAGCAGCTATTTAAATTGGTATCAGCAAAGACCAGGGAAAGCCCCTGAGCTCCTGATCTATGAAGCATA8760.10470853733170135No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG8750.10458900703794369No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT8720.10423041615667075No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT8720.10423041615667075No Hit
CAGTTTGCAAAGCGGGGTCTCATCAAGGTTCAGTGGCAGTGGATCTGGGA8690.1038718252753978No Hit
CCCCTGAGCTCCTGATCTATGAAGCATACAGTTTGCAAAGCGGGGTCTCA8580.10255699204406366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)