FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522921
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAT200.005263414756.3206568
TGGTATC2850.049.2943532
GTATCAA25500.043.0081751
GGTATCA9600.041.412171
GTGGTAT3550.038.6164931
TATCAAC30200.036.648292
AACGCAG32750.033.8993876
TCAACGC33250.033.286574
ATCAACG33200.033.233493
ACGCAGA33550.032.9889267
CAACGCA33900.032.6483355
CGCAGAG34550.032.1332868
CAGAGTA34900.031.71284910
TCGTATG553.1999452E-431.45430640
AGAGTAC35600.031.08928111
GCAGAGT35850.030.9680619
ACGGGAT1850.029.64076816
CGGGATA605.6324067E-428.56011817
TCACGAT755.82981E-527.82216644
AGTACGG32600.027.75412613
Produced by FastQC (version 0.11.5)