FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379604
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.041.461511
GTATCAA15400.038.5759121
TATCAAC17400.034.5253222
GTGGTAT2350.034.4720881
GAGTCGA450.003221358433.0081865
TGGTATC2550.031.764212
CAACGCA18950.031.5232545
ATCAACG18850.031.5114443
TCAACGC18850.031.5114444
CGAGCGA450.00434875231.04640645
CGTATGC450.00455262330.75711441
AACGCAG19450.030.7128896
ACGCAGA20350.029.354587
CGCAGAG21300.028.0453368
TCGTATG500.00768348727.63289340
AGAGTAC22250.026.99957811
TACTAAT500.008603875526.9995782
GCAGAGT22300.026.7876999
CAGAGTA22600.026.58144210
AGTACGG18200.024.29220213
Produced by FastQC (version 0.11.5)