FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671790
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10100.043.5939251
GTATCAA21850.036.838491
GTGGTAT3550.036.820751
TATCAAC25300.032.35652
TGGTATC4050.031.4232982
TCAACGC25850.031.4019414
ATCAACG26100.031.1011583
CAACGCA26100.030.9693745
AACGCAG26400.030.7477346
CGCGGTA802.605144E-630.41100543
ACGCAGA28150.028.9519637
CGCAGAG29000.028.221958
AGAGTAC29150.027.95875511
CAGAGTA30500.026.94673310
GTACACT658.807568E-426.4582864
GCAGAGT31550.025.831949
GCGGTAC959.880416E-625.62083244
TAACGGC951.0454089E-525.43514436
AGTACGG24400.025.36826513
GAGTACG24700.025.06014612
Produced by FastQC (version 0.11.5)