FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671790
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7000.10419922892570596No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAT200.00684455452.70472756
GTATCAA22600.041.2089081
GGTATCA9300.038.4309041
TATCAAC26050.035.980262
TCAACGC25750.035.9699754
ATCAACG25900.035.6398123
CAACGCA26400.035.0817385
AACGCAG26850.034.4912076
ACGCAGA28550.032.557587
CGCAGAG29950.031.0356888
AGAGTAC29600.030.93915711
CAGAGTA30950.029.92209610
GCAGAGT31550.029.7879189
CGGCAAA1055.1051925E-429.04125670
GTGGTAT3700.028.7932151
AGTACGG25800.026.72177513
TACACCG907.2158255E-626.6794435
TGGTATC3750.026.5507072
GAGTACG26300.026.34417212
TACGGGG11050.024.83228315
Produced by FastQC (version 0.11.5)