FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902367
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28210.3126222479323823No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20890.23150226016687225No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18610.20623537873171335No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18530.20534882148837444No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17810.1973698062983243No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17700.1961507900887333No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17640.19548587215622912No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17410.19293702008162975No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17330.19205046283829086No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT17100.18950161076369149No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16840.18662029972284006No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16090.1783088255665378No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTATCAGTTATTTAGCCTGGTATCAGCAAAAA15810.17520587521485162No Hit
ATGCTACATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA15740.17443013762693005No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT15350.1701081710656529No Hit
GGACTGAACAGAGAGAATTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT14890.16501046691645416No Hit
TCTTAATAGTTACCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14720.16312653277435898No Hit
CTATTAAGATGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTGTGAGAGTGAATTCTGTC14700.16290489346352427No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14480.16046686104434227No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14440.1600235824226728No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14190.15725309103723872No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14100.15625571413848247No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13620.15093637067844903No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13300.14739014170509337No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13250.14683604342800657No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13230.14661440411717183No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12560.13918948720420848No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12510.13863538892712166No Hit
GGCCAAAAGTGTACGGAGGGTAACTATTAAGATGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCG12430.13774883168378274No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11910.13198620960207988No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11770.13043473442623676No Hit
GTTGTAATGGTGCCAGCTCCTATCTTTGACCAGTGGGGCCAGGGAACCCT11740.13010227545998468No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC11710.1297698164937326No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11450.12688850545288113No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11220.12433965337828178No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11140.12345309613494289No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11070.12267735854702133No Hit
GGGTAACTATTAAGATGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTG10870.12046096543867407No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10820.11990686716158724No Hit
GATCTATGCTACATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA10720.1187986706074136No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10700.1185770312965789No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10160.11259276990404127No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10070.11159539300528498No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9980.11059801610652871No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9690.10738424609942519No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9650.10694096747775574No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9640.10683014782233836No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTAGAGGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG9590.10627604954525155No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT9480.10505703333566054No Hit
GATCAGGACTGAACAGAGAGAATTCACCATGGAGTTTGGGCTGAGCTGGC9430.10450293505857373No Hit
TAGTTACCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT9290.10295145988273063No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9120.10106752574063546No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9100.10084588642980073No Hit
ATGTAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGATAATGCC9090.10073506677438337No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTT200.00708184852.25399854
GTATCAA16800.038.843051
TCAACGC18900.034.527154
CAACGCA19150.034.2567065
TATCAAC19100.034.165612
ATCAACG19200.033.9876633
AACGCAG19550.033.55586
AGGCTCG750.003513390732.43155370
CGCAGAG21250.030.8713388
CAGAGTA21600.030.53095210
AGAGTAC21400.030.49360511
CGTATGC802.663166E-630.3272141
GCAGAGT22600.029.1800279
CGAACTA605.377167E-428.79017629
TCGTATG854.290876E-628.52580340
TGCGATA500.0076960927.62784623
GTATATA500.0077044227.6217211
ACGAGAC1057.61318E-726.31223322
AGTACGG20100.026.11008513
CTTATAC1359.34233E-925.5756681
Produced by FastQC (version 0.11.5)