FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632603
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25890.40926141671791No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20140.3183671275665781No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19910.3147313560005248No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17760.2807447957091572No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17560.277583255216937No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15280.2415416936056263No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15060.23806399906418402No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14870.23506053559657478No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14490.22905360866135632No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13730.21703975479091941No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13570.21451052239714322No Hit
GTCTGGAGCCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGAAGCC12910.20407743877281642No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12900.2039193617482054No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGC12450.19680589564070988No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12300.1944347402715447No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11720.18526627284410602No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATCATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG11720.18526627284410602No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11060.17483318921977922No Hit
GGGCATCACCGGGCTCCAGACTGGGGACGAGGCCGATTATTTCTGCGGAA10930.17277818789983607No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10550.1667712609646176No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10480.1656647217923405No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10290.1626612583247313No Hit
GTGATGCCCAGGGTGGCTGACGTGCCAGACTTGAAGCCAGAGAATCGGTC10170.1607643340293992No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10010.158235101635623No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9910.15665433138951285No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG9710.15349279089729262No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9460.14954086528201732No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9390.14843432610974022No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGCAGGTCACCATCTCCTGCTCTGG9330.14748586396207417No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9080.14353393834679887No Hit
GGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACA9060.14321778429757684No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9040.1429016302483548No Hit
GCCCCCAAACTCCTCATTTATGACAATCATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTTCAAGT9030.1427435532237438No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8820.13942393570691256No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8760.13847547355924647No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8380.13246854662402802No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8360.13215239257480602No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8210.12978123720564083No Hit
GCTCCAGACTGGGGACGAGGCCGATTATTTCTGCGGAACATGGGATAGCAGCCTGAGTTCTGTGATATTCGGCGG8070.12756815886108666No Hit
ATCCCATGTTCCGCAGAAATAATCGGCCTCGTCCCCAGTCTGGAGCCCGG8020.1267777737380316No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7980.12614546563958756No Hit
CTCCTGCTCTGGAAGCAGCTCCAACATTGCGAATAATTATGTATCCTGGTACCAGCAACTCCCAGGAACAGCCCC7980.12614546563958756No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7950.1256712345657545No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7880.12456469539347742No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7870.12440661836886642No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCGCCGCCGAATATCACAGAACTCAGGCTGCTATCCCATGTT7820.12361623324581136No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7770.12282584812275629No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7350.1161866130890938No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT7330.11587045903987178No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7310.11555430499064975No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7270.11492199689220568No Hit
GGACAGCAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGCGAATAATTATGTATCCTGGTACCAGCAA7230.11428968879376164No Hit
GGGTGGCTGACGTGCCAGACTTGAAGCCAGAGAATCGGTCAGGAATCCCT7210.11397353474453963No Hit
GGATAGCAGCCTGAGTTCTGTGATATTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC7080.11191853342459647No Hit
GCCCCAGGACAGCAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGCGAATAATTATGTATCCTGGTAC6990.11049584020309737No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6960.11002160912926431No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG6910.10923122400620926No Hit
ACATGGGATAGCAGCCTGAGTTCTGTGATATTCGGCGGCGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG6830.10796660780932117No Hit
GTTTGGGGGCTGTTCCTGGGAGTTGCTGGTACCAGGATACATAATTATTC6800.10749237673548814No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA6750.10670199161243307No Hit
GTAATAAAAGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGG6750.10670199161243307No Hit
GTTCCTGGGAGTTGCTGGTACCAGGATACATAATTATTCGCAATGTTGGA6730.10638583756321104No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG6680.105595452440156No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA6670.10543737541554499No Hit
GCCCAGGGTGGCTGACGTGCCAGACTTGAAGCCAGAGAATCGGTCAGGAA6640.10496314434171194No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6640.10496314434171194No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6590.10417275921865687No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC6580.10401468219404586No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG6530.10322429707099082No Hit
GCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGA6510.10290814302176879No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6470.10227583492332475No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG6440.10180160384949169No Hit
CTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACC6410.10132737277565866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGTT200.007361779551.74241311
GGTATCA5150.045.550321
GTATCAA14500.043.7763521
GTGGTAT1600.040.965981
TCAACGC16750.037.8929524
ATCAACG16750.037.687013
CAACGCA16800.037.574855
AACGCAG17200.036.701016
CGCAGAG17900.035.0702938
TGGTATC1800.034.494942
TATTAGA709.738196E-734.4949382
AGAGTAC18200.034.3054111
TATCAAC18900.034.1299132
ACGCAGA18600.033.9358867
CAGAGTA18500.033.93556210
AGCGTCA851.2085184E-732.4658283
GCAGAGT19450.032.275499
ATTAGAG751.669574E-632.195283
GCCTAGA553.2566753E-431.3615151
CGTTTCA450.004626241530.65974618
Produced by FastQC (version 0.11.5)