FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574823
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28450.044.036231
GTATCAA79350.042.6593481
TGGTATC8850.039.1311762
GTGGTAT9400.038.2863541
TATCAAC90450.037.6494262
ATCAACG95250.035.680843
TCAACGC96700.035.1458134
CAACGCA98250.034.6259085
AACGCAG99250.034.3796586
ACGCAGA104450.032.6628957
CGCAGAG105000.032.4594738
AGAGTAC108000.031.68355211
CAGAGTA109100.031.30186810
GCAGAGT111500.030.5976629
TCGACGT1900.030.43226829
TCGTATG1850.029.81651740
CGTATGC2050.028.65491741
AGTACGG85400.027.7456313
GAGTACG86100.027.55948312
TACGGGA21500.027.15905415
Produced by FastQC (version 0.11.5)