FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574823
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23250.14763563905276975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC252.8154263E-454.17924510
GGTATCA29650.044.868411
GTATCAA78450.041.3132861
TGGTATC8650.040.3635522
GTGGTAT9100.039.904481
TATCAAC89750.035.880412
ATCAACG96200.033.48443
TCAACGC97050.033.147814
CAACGCA99150.032.5130125
AACGCAG100750.032.1647266
ACGCAGA105650.030.6729417
CGCAGAG105800.030.6294568
AGAGTAC108600.029.96446811
CAGAGTA110250.029.45459210
GCAGAGT112900.028.7932229
AGTACGG88350.026.40740213
GAGTACG89300.026.0885512
ACGCTAA1250.001214903425.04005470
ACGGGTA12000.024.83846516
TACGGGT44250.024.18323315
Produced by FastQC (version 0.11.5)