FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499457
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13850.040.4257771
GTGTTAC801.708031E-938.6431961
GGTATCA5950.035.7927671
CTACCGT400.002656376234.3357428
TCAACGC16500.033.8821954
ATCAACG16550.033.7899783
TATCAAC16700.033.702042
CAACGCA16650.033.576955
AACGCAG17050.032.9903836
ACGCAGA18150.031.1799347
CGCAGAG18550.030.5075938
CGACCGT450.004754896730.48704714
CAGAGTA19150.029.37263710
AGAGTAC18900.029.21675311
GCAGAGT19850.028.5096119
CGTAGGT500.007455476627.8057843
TACCGTT500.00788588127.48787529
ACTACCG500.00792067827.46308527
CGCCGTA500.00793618227.45208425
AGTACGG16000.026.1521713
Produced by FastQC (version 0.11.5)